2 R P T P A B Hg(II) Abb.2 Regulator 144 aa Promotor Transport Peri- plasma116 aaTransportMembrane91 aaMercury-Reductase561 aaMercury-Lyase212 aaAbb.2
3 Operon mRNA Metabolite P Enzyme Abb. 3 RG Promotor Operator TF Struc. DNARepressorApo-repressorTranscriptionmRNADegradationFeedbackrepressionInhibitionTranslationMetabolite PEnzymeSecretionDegradationThe production of metabolites is determined at the end by gene expression. The interactions of gene expression with cellular metabolism are an important issue in many fields including metabolic engineering. The metabolites can interact with almost all the essential steps on the path from DNA to metabolites such as transcrption, translation and enzymatic catalysis. Of particular relevance are feedback repression of the operator activity, inhibition of enzyme activities and global regulation. There are also several regulatory genes coded in the near of the structure genes. Together with the promoter and operator these regulatory and structure genes form a genetic unit called operon or regulon. We have mathematically modeled and analyzed the operons for propanediol formation and tryptophan biosynthesis. I am not going to talk about the models in detail. In principle, for each of the steps from DNA to metabolite P, such a differential equation can be formulated.The mathematic modeling can help to assess the relative importance of these regulation mechanisms and to formulate experimental hypothesis.Abb. 3
5 The interactions of gene expression and cellular metabolism can lead to strong nonlinear dynamics of cells such as oscillations and multiplicity. This graph shows for example oscillations of mRNA, enzyme and tryptophan concentrations in the tryptophan biosynthesis under certain conditions. The study of cellular dynamics requires the expertise of engineers in system analysis and dynamic modeling and is also within my research interest.Abb. 5
6 2-D IEF/SDS-PAGE Gel Electrophoretic Separation Abb. 6
11 Nahrung = 350 Signale A B Abb. 11 pH-Wert Osmolarität Temperatur Gen mRNA ProteinTemperaturAntibiotikaSauerstoffABGift/GefahrLichtErdanziehungskraftNährstoff-limitierungBotschaften andererBakterienScherstressDruckAbb. 11
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