Karin Kovar – Verena Looser – Iwo Zamora Das bewegte Papier Karin Kovar – Verena Looser – Iwo Zamora 1 1

papierlos = digital (& virtuell) Ablage im Moodle ‘neue’ Didaktik NEULAND VS. 2 2

Erfahrungsbericht Simulation Was ist «papierlos»? Erfahrungsbericht Simulation Analogie Dialog

microbial physiology (ecology) Wädenswil Bioprocess Technology & Informatics (since 1999) microbial physiology (ecology) engineering data ‚mining‘

bacteria microalgae E. coli Chlorella Bacillus Scenedesmus etc. Galdieria Trachydiscus etc. Pichia pastoris Saccharomyces Hansenula Yarrowia yeast 5 5

Prozessdaten # 174 Prozesse and # 97 Variablen

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16 cells 64 cells 512 cells 256 cells 8 cells 2 cells 4 cells 1 cell 9 9

26 cells 27 cells 28 cells 210 cells 24 cells 25 cells 20 cells

Simulation 1, 2, 4, 8, 16, 32, 64, 128, 256... …10x 20, 22, 23, 24, 25, 26, 27... ….2n 11 11

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didaktisches Szenario angewandte Datenanalyse (angewandte) Mathematik Biochemie Biotechnologie Ingenieurwesen Verfahrenstechnik Molekularbiologie Physiologie T R S E Theorie – Rechenübungen – Simulation – Experiment

Bachelor = Praxis 3 2

Erfahrungsbericht Simulation Was ist «papierlos»? Erfahrungsbericht Simulation Analogie Dialog

hands-on laboratory experiments

hands-on laboratory experiments

hands-on laboratory experiments

hands-on laboratory experiments

hands-on laboratory experiments

Mitdenken & Notieren (Moodle in der Regel…)

Substrat wird verbraucht. Biomasse wird gebildet.

Das «Pulver»… …wird zu Biomasse.

What-if

Simulation

- -- - -- ++ + ++ + Evaluation Blockkurs Vorlesung & Praktikum Basel Waedenswil - -- - -- ++ + ++ + n = 31 n = 33 Die PC-Übungen haben mir geholfen, den Vorlesungsinhalt zu begreifen. Die Arbeit mit der Simulation hat dazu beigetragen, dass ich den vorgelesenen Stoff besser begriffen habe. Die Aufgabe ist sehr aufwändig, aber sinnvoll.

Was ist «papierlos»? Swiss Virtual Campus www.biotechLAB.net

further prospects – biotechLAB

Erfahrungsbericht Simulation Was ist «papierlos»? Erfahrungsbericht Simulation Analogie Dialog

qp = f(qs, µ, etc.) ?

Computer: Berechnungen Optimierung Labor: Datenerhebung Computer: Berechnungen Mensch: heuristisches Wissen 31 31

Optimierung μopt ? qp,max 32 32

Analogien Beutelmull Goldmull Gürtelmull ? 33 33

Analogien P. pastoris Organismus? μopt qp,max 34 Variante 1 Variante 2

Verifizierung 35 35

Verifizierung 36 36

Simulationen 37 37

NBO FBaid µoptimal µ - control in fedbatch Most biotechnological manufacturing processes are carried out in fedbatch mode Majority of industrial relevant products have a partly growth linked product formation Screening / early stage development has to be done under production conditions (Lattermann & Büchs, 2015)  product development specialists have to be able to do fedbatchs Generally the most biotechnological manufacturing processes are carried out in fedbatch mode Additionally have the majority of industrial relevant products a partly growth linked product formation. That means the productivity of an organism is linked with it’s specific growth rate.

Bench-top bioreactors NBO FBaid Shake flasks Micro-bioreactors ambr15™ Bench-top bioreactors Of course there are already different tools on the market to do fedbatch cultivations in a easy or automated way. But most of the tools haven’t the possibility to really control the specific growth rate of the production organism like shake flasks with enzymatically controlled substrate release. You can reach higher biomass- and product concentrations but you wont be able to control the specific growth rate. In the case of micro-bioreactors the addition of substrate ist done with pulses, that means you have like repeated batch cultivations so they growing with maximum speed until the substrate is consumed and then they don’t growt until the next substrate addition is happening and they grow with maximum speed again. There are also such parallel bioreactor systems like this ambr15 with a higher bioreactor volume. In this systems are real Fedbatch processes possible but they are very very expensive. Our conclusion was that the best alternative would be relatively priceless benchtop reactors with a software tool which guides the user through the preparation, cultivation and data evaluation of a Fedbatch process. To make a long story short: the outcome of our NBO report was, that such a software tool itselfe wouldn’t be feasible. But if we would cooperate with a reactorbulding company which integrates the tool in the already existing process control system. The market access would be better and it could be a feasible business case. For the cooperation we identified Infors HT as a good partner. Because they are a swiss reactor building company and there were already some contacts established between Karin Kovar an Infors HT Enzymatically controlled substrate release High-throughput screening in micro-, mili-scale Software computational approach to control conversion rates

Concept/Idea of my NBO Commercial software that guides users through the analysis of data gained from bioprocesses The question of my NBO was if a it solution which

Lehren wir, was wir forschen? Forschen wir, was wir lehren? Was ist «papierlos»? Lehren wir, was wir forschen? Forschen wir, was wir lehren?

Erfahrungsbericht Simulation Was ist «papierlos»? Erfahrungsbericht Simulation Analogie Dialog

Why is a disciplined approach to NBD so critical? raw ideas (3,000) ideas submitted (300) small projects (125 vs. 22) early stage development (9) major development (4) launches (1.7) market success (1) Stevens and Burley, 1997. 3000 Raw Ideas = 1 Commercial Success!, Research Technology Management, 40(3): 16-27

questions #3 – course content What should the students’ analysis do? Describe the current, specific state-of-the-art including a comparison with product and technology alternatives.  Provide an assessment of the novelty of the product or technology and a summary of the intellectual property landscape. Provide an estimate of the total market potential for the product or technology including an assessment of the influence of competitive products and an indication of the expected impact of the product or technology on the value chain. Draw conclusions on the state-of-the-art of the science/technology and the likelihood of commercial success of the technology and outline recommendations for the “path forward”.  Highlight additional product or technology development opportunities and opportunities for further innovation. Impress their stakeholders through the quality and thoroughness of the analysis.

course objectives (biotechnology – business – skills) future trends in bio-process technology innovation (molecule, indication, organism, technology…) new business development (best practices & tools) analysis (critical thinking, incomplete information, etc.) information – knowledge – decision

biofuels microalgae (Chlorella sp.) bio-fuels

2010: 630 microalgae (10% reactor) (fossil) crude oil price science vs. economics 2010: 630 microalgae (10% reactor) (fossil) crude oil price # publications

crude oil price * * * Mars mission 1 2 3 4 5 1 2nd oil crisis 2 Iran-Iraq war 3 Gulf war 4 financial crisis 5 EU dept crisis * Kyoto Protocol * Mars mission 1987-1998 Aquatic Species Program (US) since 2008 Bundesalgenstammtisch (D) 2011-2022 Biomass Program (US) Japanese ‘Programs’ Source: Capital Professional Services, LLC, Updated May 13, 2011; http://inflationdata.com/inflation/inflation_rate/historical_oil_prices_table.asp Prices are adjusted for Inflation to April 2011 prices using the Consumer Price Index (CPI-U) as presented by the Bureau of Labor Statistics

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  mutual benefits etc.

ZHAW-Jahresbericht 2015, Seite 13

papierlos + beweglich + interaktiv + selbstbestimmend + aufwendig Was ist «papierlos»? papierlos + beweglich + interaktiv + selbstbestimmend + aufwendig

course structure steps of knowledge acquisition methods i a e p d i a d - design of experiment (& hypothesis) p - preparation e - laboratory experiment a - data analysis i - interpretation/conclusion d - design of experiment e p d SL i a M e SC p d methods SC CYCLICAL SUBJECT MATTER acquisition SL LOGICAL SUBJECT MATTER acquisition M METHODICAL acquisition

valuable painting in a suitable frame… …we are improving 60 60