26 - -- - -- ++ + ++ + Evaluation Blockkurs Vorlesung & Praktikum BaselWaedenswil------++++++n = 31n = 33Die PC-Übungen haben mir geholfen,den Vorlesungsinhalt zu begreifen.Die Arbeit mit der Simulation hat dazubeigetragen, dass ich denvorgelesenen Stoff besser begriffen habe.Die Aufgabe ist sehr aufwändig, aber sinnvoll.
38 NBO FBaidµoptimalµ - control in fedbatchMost biotechnological manufacturing processes are carried out in fedbatch modeMajority of industrial relevant products have a partly growth linked product formationScreening / early stage development has to be done under production conditions (Lattermann & Büchs, 2015) product development specialists have to be able to do fedbatchsGenerally the most biotechnological manufacturing processes are carried out in fedbatch modeAdditionally have the majority of industrial relevant products a partly growth linked product formation. That means the productivity of an organism is linked with it’s specific growth rate.
39 Bench-top bioreactors NBO FBaidShake flasksMicro-bioreactorsambr15™Bench-top bioreactorsOf course there are already different tools on the market to do fedbatch cultivations in a easy or automated way. But most of the tools haven’t the possibility to really control the specific growth rate of the production organism like shake flasks with enzymatically controlled substrate release. You can reach higher biomass- and product concentrations but you wont be able to control the specific growth rate. In the case of micro-bioreactors the addition of substrate ist done with pulses, that means you have like repeated batch cultivations so they growing with maximum speed until the substrate is consumed and then they don’t growt until the next substrate addition is happening and they grow with maximum speed again. There are also such parallel bioreactor systems like this ambr15 with a higher bioreactor volume. In this systems are real Fedbatch processes possible but they are very very expensive. Our conclusion was that the best alternative would be relatively priceless benchtop reactors with a software tool which guides the user through the preparation, cultivation and data evaluation of a Fedbatch process. To make a long story short: the outcome of our NBO report was, that such a software tool itselfe wouldn’t be feasible. But if we would cooperate with a reactorbulding company which integrates the tool in the already existing process control system. The market access would be better and it could be a feasible business case. For the cooperation we identified Infors HT as a good partner. Because they are a swiss reactor building company and there were already some contacts established between Karin Kovar an Infors HTEnzymatically controlled substrate releaseHigh-throughput screening in micro-, mili-scaleSoftware computational approach to control conversion rates
40 Concept/Idea of my NBOCommercial software that guides users through the analysis of data gained from bioprocessesThe question of my NBO was if a it solution which
42 Lehren wir, was wir forschen? Forschen wir, was wir lehren? Was ist «papierlos»?Lehren wir,was wir forschen?Forschen wir,was wir lehren?
43 Erfahrungsbericht Simulation Was ist «papierlos»?Erfahrungsbericht SimulationAnalogieDialog
44 Why is a disciplined approach to NBD so critical? raw ideas (3,000)ideas submitted (300)small projects (125 vs. 22)early stage development (9)major development (4)launches (1.7)market success (1)Stevens and Burley, Raw Ideas = 1 Commercial Success!, Research Technology Management, 40(3): 16-27
45 questions #3 – course content What should the students’ analysis do?Describe the current, specific state-of-the-art including a comparison with product and technology alternatives. Provide an assessment of the novelty of the product or technology and a summary of the intellectual property landscape.Provide an estimate of the total market potential for the product or technology including an assessment of the influence of competitive products and an indication of the expected impact of the product or technology on the value chain.Draw conclusions on the state-of-the-art of the science/technology and the likelihood of commercial success of the technology and outline recommendations for the “path forward”. Highlight additional product or technology development opportunities and opportunities for further innovation.Impress their stakeholders through the quality and thoroughness of the analysis.
46 course objectives (biotechnology – business – skills) future trends in bio-process technologyinnovation (molecule, indication, organism, technology…)new business development (best practices & tools)analysis (critical thinking, incomplete information, etc.)information – knowledge – decision
49 crude oil price * * * Mars mission 1 2 3 4 5 1 2nd oil crisis 2 Iran-Iraq war3 Gulf war4 financial crisis5 EU dept crisis* Kyoto Protocol* Mars missionAquatic Species Program (US)since 2008Bundesalgenstammtisch (D)Biomass Program (US)Japanese ‘Programs’Source: Capital Professional Services, LLC, Updated May 13, 2011; Prices are adjusted for Inflation to April 2011 prices using the Consumer Price Index (CPI-U) as presented by the Bureau of Labor Statistics
56 papierlos + beweglich + interaktiv + selbstbestimmend + aufwendig Was ist «papierlos»?papierlos+ beweglich+ interaktiv+ selbstbestimmend+ aufwendig
57 course structure steps of knowledge acquisition methods i a e p d i a d - design of experiment (& hypothesis)p - preparatione - laboratory experimenta - data analysisi - interpretation/conclusiond - design of experimentepdSLiaMeSCpdmethodsSCCYCLICAL SUBJECT MATTER acquisitionSLLOGICAL SUBJECT MATTER acquisitionMMETHODICAL acquisition