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Institut für Hochfrequenztechnik Technische Universität Braunschweig Department of Biological and Medical Psychology Non invasive imaging of plants and.

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Präsentation zum Thema: "Institut für Hochfrequenztechnik Technische Universität Braunschweig Department of Biological and Medical Psychology Non invasive imaging of plants and."—  Präsentation transkript:

1 Institut für Hochfrequenztechnik Technische Universität Braunschweig Department of Biological and Medical Psychology Non invasive imaging of plants and plant roots in substrate with Nuclear Magnetic Resonant Imaging (NMRI) and (Sub) Terrahertz Imaging (THz) Dr. Axel Jung Ricarda van der HeijdenBeta Klinik Bonn Dr. Karsten SpechtUniversity of Bergen Prof. Dr. Martin KochIHF TU Braunschweig Dr. Jörg Vandenhirtz Matthias Eberius Dirk VandenhirtzLemnaTec

2 Institut für Hochfrequenztechnik Technische Universität Braunschweig Department of Biological and Medical Psychology LemnaTec High Throughput Phenotyping Platform

3 Institut für Hochfrequenztechnik Technische Universität Braunschweig Department of Biological and Medical Psychology Goal of this joint study: Can Nuclear Magnetic Resonant Imaging (NMRI) or (Sub) Terrahertz Imaging (Thz) be used for obtaining non invasive and valuable information about plant roots in substrate? 3D root morphologie 3D root dynamics 3D root activity (water distribution) etc...

4 Institut für Hochfrequenztechnik Technische Universität Braunschweig Department of Biological and Medical Psychology Used Plants: Corn Plants in transparent pots:

5 Institut für Hochfrequenztechnik Technische Universität Braunschweig Department of Biological and Medical Psychology Siemens MAGNETOM Espree 1.5T NMR settings: * B = 1.5 T TE = 12 ms TR = 5.0 s FoV = 250 x 250 ST = 1 mm * Dr. Peter Blümeler FZ Jülich ICG-3 ecoNMR Magnetic Resonance of plants Price: 1.5 Mio Euro

6 Institut für Hochfrequenztechnik Technische Universität Braunschweig Department of Biological and Medical Psychology First Results of Dr. Axel Jung, Beta-Klinik, Bonn

7 Institut für Hochfrequenztechnik Technische Universität Braunschweig Department of Biological and Medical Psychology First Results of Dr. Axel Jung, Beta-Klinik, Bonn

8 Institut für Hochfrequenztechnik Technische Universität Braunschweig Department of Biological and Medical Psychology Problems: artefacts (debris) in substrate hide roots (H2O + Fe) low contrast between roots and background correct setting of NMR has to be researched -> Contact to Dr. Karsten Specht Department of Biological and Medical Psychology University of Bergen, Norway Research on NMRI Dr. Specht tried NMRI of his office Plant...

9 Institut für Hochfrequenztechnik Technische Universität Braunschweig Department of Biological and Medical Psychology First Results of Dr. Karsten Specht, Uni Bergen: CONFIDENTIAL

10 Institut für Hochfrequenztechnik Technische Universität Braunschweig Department of Biological and Medical Psychology First Results of Dr. Karsten Specht, Uni Bergen: CONFIDENTIAL

11 Institut für Hochfrequenztechnik Technische Universität Braunschweig Department of Biological and Medical Psychology NMRI Conclusions: + Standard medical NMR scanners are able to provide 3D information about root architecture and dynamics with mm resolution + NMRI is a promising tool for non invasive root imaging of plants o Correct NMR settings for root imaging have to be researched and optimized o Peat substrate minimizes H2O and Fe background problems - Measurement time (ca. 20 min.) not suitable for high throughput - Very expensive (ca. 1.5 Mio Euro)

12 Institut für Hochfrequenztechnik Technische Universität Braunschweig Department of Biological and Medical Psychology Complementary option for non invasive and penetrating plant sensors: Prof. Dr. Martin Koch Institut für Hochfrequenztechnik TU Braunschweig THz radation can be considered as high-frequency microwaves or very long waved light.

13 Institut für Hochfrequenztechnik Technische Universität Braunschweig Department of Biological and Medical Psychology Water concentration Fresh leaf 48 hours after it has been cut out B.B. Hu und M.C. Nuss, Opt. Lett. 20, 1716 (1995) First THz image of a leaf with high resolution Bell Labs Lucent Technologies CONFIDENTIAL

14 Institut für Hochfrequenztechnik Technische Universität Braunschweig Department of Biological and Medical Psychology CONFIDENTIAL First Results of Prof. Dr. Martin Koch, Braunschweig: THz transmitter THz detector moving a coffee plant between a sub THz transmitter and detector… …and measuring the transmission of the sub THz signal

15 Institut für Hochfrequenztechnik Technische Universität Braunschweig Department of Biological and Medical Psychology The lift and rotating devices of the LemnaTec Phenotyping Platform are already integrated for 3D THz scans in cylindrical coordinates: z THz Combining 3D THz transmission measurements with visible 3D RGB image data of the LemnaTec Phenotyping Platform transmitterdetector

16 Institut für Hochfrequenztechnik Technische Universität Braunschweig Department of Biological and Medical Psychology THz Conclusions: + Measurement time (ca. 2 min.) suitable for high throughput + Cheaper as NMRI (ca. 150,000 Euro) + THz is a promising alternative for non invasive 3D imaging of plants + plastic is tranparent for THz radiation -> plastic hydroponics could be used for THz root imaging o Hardware and software for THz root imaging have to be researched and optimized - Resolution 1 cm not suitable for root architecture

17 Institut für Hochfrequenztechnik Technische Universität Braunschweig Department of Biological and Medical Psychology Conclusions: NMRITHz Hardware standard of the shelf technologyto be developed Software to be developed Throughput ca. 20 min. per plantca. 2 min. per plant Resolution ca. 1 mmca. 1 cm Hazards high B-Fieldnone Price ca. 1.5 Mio. Euroca. 150,000 Euro Target roots and shootsshoots (roots?)


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